Journal:
Article Title: Murine Cytomegalovirus m02 Gene Family Protects against Natural Killer Cell-Mediated Immune Surveillance
doi: 10.1128/JVI.76.2.885-894.2002
Figure Lengend Snippet: m02 gene family is not required for MCMV growth in cultured fibroblasts or macrophages. 10.1 or IC21 cells were infected at the indicated multiplicities of infection (moi) with the wild-type (▪), SMsubm02 (⧫) (A), or SMsubm02-16 (▴) (B) virus. Supernatants were harvested at the indicated times, and virus titers were quantified by plaque assay on 10.1 cells. Three independently infected cultures were assayed for each time point.
Article Snippet: Viral growth also was also examined in the IC21 mouse macrophage cell line (ATCC TIB-186), which was propagated in RPMI-1640 medium (ATCC 30-2001) supplemented with 10% fetal bovine serum.
Techniques: Cell Culture, Infection, Virus, Plaque Assay